Comparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria
نویسندگان
چکیده
منابع مشابه
Comparative Study of 14C-Labeled Purified Protein Derivative from Various Mycobacteria
A study on skin cross-reactivity between stabilized "4C-labeled mycobacterial antigens, namely tuberculin purified protein derivative (PPD; from Mycobacterium tuberculosis), PPD-A (M. avium), PPD-Y (M. kansasii), PPD-G (M. scrofulaceum), PPD-B (M. intracellulare), and PPD-F (M. fortuitun), has been carried out in groups of guinea pigs sensitized with one of the following heat-killed mycobacteri...
متن کاملComparative study of 14C-labeled purified protein derivative from various mycobacteria. I. Preparation of 14C-labeled purified protein derivative antigens and their adsorption to glass.
Biologically active (14)C-labeled purified protein derivative ((14)C-PPD) has been prepared from the culture filtrates of seven species of mycobacteria, namely Mycobacterium tuberculosis Johnston strain (PPD), M. bovis BCG (PPD-BCG), M. avium (PPD-A), M. kansasii (PPD-Y), M. intracellulare (PPD-B), M. scrofulaceum (PPD-G), and M. fortuitum (PPD-F). These mycobacteria were grown in a culture med...
متن کاملPreparation of 14C-labeled tuberculin purified protein derivative.
(14)C-labeled tuberculin purified protein derivative ((14)C-tuberculin PPD) has been prepared from culture filtrates of Mycobacterium tuberculosis var. hominis grown in a culture medium containing uniformly labeled (14)C-amino acids. With a mixture of (14)C-amino acids (an acid hydrolysate of (14)C-Chlorella protein) in the medium, the recoveries of (14)C in the final product were higher than w...
متن کاملPurified Protein Derivative--PPD.
A 55-year-old physician had a PPD made as part of a clinical evaluation. Laboratory tests were unremarkable and PPD, read in 48 hours, showed an area of induration of 18 mm and an erythema of 51 mm (Panel A). A 2 mm punch biopsy was done in the center of the induration 96 hours after the injection. Histopathology showed an intense perivascular cuffing with macrophages and lymphocytes and edema ...
متن کاملextraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1970
ISSN: 0003-6919
DOI: 10.1128/aem.20.5.696-703.1970